Do you know Glass Bottom Dishes? Glass Bottom Dishes are used in the fields of Life Science and Environmental Research, Molecular and Cell Biology, Cell Culture and Cell Physiology, Biotechnology, Neurology, Pathology, Pharmacology and more.
These sterile tissue culture grade polystyrene glass bottom WillCo Wells Petri dishes with lid are available in two sizes, with clear or black plastic sidewalls. Through their advanced design features, they provide exceptional image quality needed for use on inverted microscopes for applications such as high resolution image analysis, microinjection and electro-physiology recording of fluorescent-tagged cells.
The glass bottom Petri dish uses a special formulated adhesive with a low toxicity that is durable and optically clear. Glass thickness stays within 0.17 ±0.01mm and permits use of immersion objectives with water, glycerin or oil. The glass bottom is flush with the microscope stage or heating stage to ensure high stability and optimum heat exchange. The optical quality of the glass bottom provides a higher effective numerical aperture producing brighter high resolution images in all types of microscopy applications.
The glass bottom petri dish features a low rim side wall. The low side wall allows easier access for micromanipulation/ microinjection probes. The Glass bottom Willco Well Petri dish is available in two sizes: 35mm diameter and 50mm diameter. Both sizes are available with clear or black side walls.
The 35mm dish has outside dimensions similar to that of a Corning 35mm dish and will be compatible with most 35mm heaters and perfusion adapters.
Advantages:
- Much larger effective range, using an objective lens with a short working distance
- No birefringence effects because of thin (0.17mm) glass bottom
- Low rim/side wall of the dish and a larger effective working distance make micromanipulation and microinjection possible
- Higher effective N.A. make brighter images (in e.g.: fluorescence)
- Higher effective N.A. make higher resolutions in image analysis
Microscope light passes through the bottom glass in a perpendicular way, glass rests on stage - Direct heating from the microscope-stage, because the glass is in direct contact (flush) with stage
- In IVF, no more oocyte-/embryo transfer is needed from one plastic dish to another, unless different media are being used, saving a lot of time
- Easy "perfusion" in the dish for continuous in and outflow of solutions, using "perfusion chamber insert"
- The glass is used in microscopy for medical, biological and research work, eg. fluorescence microscopy
Applications:
- Live Cell Microscopy
- Multi-Photon Laser Scanning Microscopy (MPLSM)
- High Level Laser Fluorescence
- Fluorescence Lifetime Imaging Microscopy (FLIM)
- Fluorescence Resonance Energy Transfer Microscopy (FRETM)
- Fluorescence Redistribution After Photobleaching Microscopy (FRAPM)
- Epifluorescence Microscopy
- Fluorescence Imaging
- Immunofluorescence Microscopy
- Confocal Microscopy
- Confocal Laser Scanning Microscopy (CLSM)
- Phase Contrast Microscopy
- Polarized Light Microscopy
- Differential Interference Contrast
- High Resolution Image Analysis
- Time Lapsed Photography
- Video-Enhanced Microscopy
- Infrared imaging
- Calcium imaging
- Colocalization studies
- Green Fluorescent Protein (GFP)
- Micromanipulation
- Microinjection