Cell culture

Product Name: Cell culture

 

Pinyin: xibaopeiyang

 

English name: culture of cells

 

Description: Includes microbial cells, plant cells and animal cell culture. The animal and plant tissue or cells removed from the body, dispersed into a single cell or directly to single-celled organisms, so that the necessary growth conditions in the culture medium or culture flask to continue growth and proliferation. Cell culture is a very important technology in cell biology research. Cell cycle and its regulation, cancer mechanism, aging, gene expression and regulation and other important progress are inseparable from cell culture technology. Plant cell culture has opened up a new way for plant breeding. Large-scale cell culture for the nutrition, vaccine production and drug research, development and prevention and treatment of cancer provides a new means. Prokaryotes, virus culture for genetic engineering and other modern biological engineering provides the necessary conditions.

 

The basics of cell culture

Cell culture The meaning of cell culture is simply to disperse the tissue from the body into individual cells and survive in an in vitro environment similar to that in the body, allowing it to grow, multiply, or pass to observe the cells The growth, reproduction, aging and other life phenomena. But also can use cells for cell engineering and cell cancer and other major issues of research. Cell culture is also the basis for researching viruses and developing vaccines. Therefore, cell culture technology in genetics, immunology, oncology, virology, molecular biology and other fields has been widely used. The following describes the basic concepts of primary culture and subculture.

 

Primary culture The monolayer cells were grown directly from the tissue mass or the tissue was dispersed into individual cells by enzymatic or mechanical methods. The culture before the first passage was considered to be primary culture. The primary advantage of primary culture is that the tissue and cells just in vitro, biological traits have not changed a lot, to a certain extent, can reflect the state of the body. Especially in the cell culture rendezvous, the primary culture of some special functional expression is particularly strong. In such a culture stage, it is possible to better show the morphological characteristics that are closely related to the body tissue. In the case of sufficient donor and biological conditions, the use of primary culture to do a variety of experiments, such as drug testing, cell differentiation, the effect is very good. However, it should be noted that the primary culture tissue is composed of a variety of cell components, more complex. Even if all of the same type of cells, such as epithelial cells or fibroblasts, are still heterogeneous, in the analysis of cell biological characteristics more difficult. Second, due to donor individual differences and other reasons, the cell population growth effect is sometimes inconsistent.

 

Primary culture is also the establishment of a variety of cell lines (strains) must pass the stage. If the primary culture can be maintained for several hours or even longer, can be further screening. Some cells have the ability to continue to proliferate, some cell types only survive without proliferation, while others are only applied under special conditions without survival, so the cell type distribution will change. In the case of monolayer culture, the bottom of the bottle is covered with cells, and after the meeting, the density-dependent cells are gradually reduced in growth, while the cells that lose density-dependent sensitivity increase, and the natural or spontaneous transformed cells Excessive growth. With frequent passage, maintaining low density growth of cells, is conducive to preserving the normal phenotype of cells (such as mouse fibroblasts). While spontaneous transformation tends to excessive growth of high cell density.

 

Subculture after primary culture cells form confluent monolayer, need to be isolated and cultured, otherwise the cells will generate due to lack of space or because of cell density caused by excessive nutrient depletion, will affect the growth of cells, a procedure often referred to passages or Subculture. Primary culture in the first passage is the cell line, can be continuously cultured for continuous cell lines; can not be continuously cultured for the limited cell line. Usually, subculture refers to the expansion of culture, that is, a cell is divided into two or one into three for culture. But strictly speaking, regardless of dilution or not, transfer cells from one flask or transplant to another culture flask, known as passaged or subcultured. It is understandable that at any time the cells will always lose a portion when they are inoculated from one bottle to another, so that the cells must be diluted in an objective manner.

 

The concept of passage algebra is often confused with "proliferation algebra". The term & quot; generation & quot; of cells refers only to a period of time from cell inoculation to separation and re-culture. Such as a cell line for the 153th generation, which means that the cell line has been passed 153 times. It is different from the cell "proliferation algebra" (cell generation or multiplication), in the cell generation, the cells can be doubled by 3 to 6 times. It can be seen that the cell algebra is related to the proliferation algebra, and the exact algebra depends on the cell strain and the culture conditions. But the growth conditions that make up a cell line must be the same, so the cell line should express an approximate multiplication or multiplication algebra.

 

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